US Central Command said it fired at more than 80 Iranian targets, including over 60 Islamic Revolutionary Guard Corps small boats.
In response, Iran said it targeted US military sites in Bahrain and Kuwait.
The US strikes followed attacks earlier in the week on three oil tankers in the Strait of Hormuz, which Iran has not directly claimed responsibility for.
The US military attacked Iran early Wednesday after it said Tehran struck three ships in the Strait of Hormuz, part of an American effort that also revoked the Islamic Republic’s ability to openly sell crude oil in the world market. Iran retaliated with strikes targeting Bahrain and Kuwait.
The regional crossfire raised the risks that an interim agreement to halt fighting in the war could break down, putting the Middle East again at risk of a wider conflict.
However, the fire followed a pattern of similar attacks during the deal’s shaky ceasefire, and neither country immediately signaled they’d leave the negotiating table.